Outlining Core Pathways of Amyloid Toxicity in Bacteria with the RepA-WH1 Prionoid
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چکیده
منابع مشابه
Outlining Core Pathways of Amyloid Toxicity in Bacteria with the RepA-WH1 Prionoid
The synthetic bacterial prionoid RepA-WH1 causes a vertically transmissible amyloid proteinopathy in Escherichia coli that inhibits growth and eventually kills the cells. Recent in vitro studies show that RepA-WH1 builds pores through model lipid membranes, suggesting a possible mechanism for bacterial cell death. By comparing acutely (A31V) and mildly (ΔN37) cytotoxic mutant variants of the pr...
متن کاملPre-amyloid oligomers of the proteotoxic RepA-WH1 prionoid assemble at the bacterial nucleoid
Upon binding to short specific dsDNA sequences in vitro, the N-terminal WH1 domain of the plasmid DNA replication initiator RepA assembles as amyloid fibres. These are bundles of single or double twisted tubular filaments in which distorted RepA-WH1 monomers are the building blocks. When expressed in Escherichia coli, RepA-WH1 triggers the first synthetic amyloid proteinopathy in bacteria, reca...
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Understanding protein amyloidogenesis is an important topic in protein science, fueled by the role of amyloid aggregates, especially oligomers, in the etiology of a number of devastating human degenerative diseases. However, the mechanisms that determine the formation of amyloid oligomers remain elusive due to the high complexity of the amyloidogenesis process. For instance, gold nanoparticles ...
متن کاملRepA-WH1 prionoid: Clues from bacteria on factors governing phase transitions in amyloidogenesis
In bacterial plasmids, Rep proteins initiate DNA replication by undergoing a structural transformation coupled to dimer dissociation. Amyloidogenesis of the 'winged-helix' N-terminal domain of RepA (WH1) is triggered in vitro upon binding to plasmid-specific DNA sequences, and occurs at the bacterial nucleoid in vivo. Amyloid fibers are made of distorted RepA-WH1 monomers that assemble as singl...
متن کاملAggregation interplay between variants of the RepA-WH1 prionoid in Escherichia coli.
The N-terminal domain (winged-helix domain, or WH1) of the Pseudomonas pPS10 plasmid DNA replication protein RepA can assemble into amyloid fibers in vitro and, when expressed in Escherichia coli, leads to a unique intracellular amyloid proteinopathy by hampering bacterial proliferation. RepA-WH1 amyloidosis propagates along generations through the transmission of aggregated particles across th...
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2017
ISSN: 1664-302X
DOI: 10.3389/fmicb.2017.00539